Occurrence of Gibberella zeae strains that produce both nivalenol and deoxynivalenol

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Tri13 and Tri7 determine deoxynivalenol- and nivalenol-producing chemotypes of Gibberella zeae.

Gibberella zeae, a major cause of cereal scab, can be divided into two chemotypes based on production of the 8-ketotrichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned and sequenced a Tri13 homolog from each chemotype. The Tri13 from a NIV chemotype strain (88-1) is located in the trichothecene gene cluster and carries an open reading frame similar to that of Fusarium sporotrichio...

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Identification of deoxynivalenol- and nivalenol-producing chemotypes of Gibberella zeae by using PCR.

Gibberella zeae, a major cause of cereal scab, may be divided into two chemotypes based on production of the trichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned and sequenced the gene cluster for trichothecene biosynthesis from each chemotype. G. zeae H-11 is a DON producer isolated from corn, and G. zeae 88-1 is a NIV producer from barley. We sequenced a 23-kb gene cluster from ...

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Natural Occurrence of Nivalenol, Deoxynivalenol, and Deoxynivalenol-3-Glucoside in Polish Winter Wheat

The presence of mycotoxins in cereal grain is a very important food safety factor. The occurrence of "masked" mycotoxins has been intensively investigated in recent years. In this study, the occurrence of nivalenol, deoxynivalenol-3-glucoside, and deoxynivalenol in 92 samples of winter wheat from Polish cultivars was determined. The frequency of the occurrence of deoxynivalenol and nivalenol in...

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Some Genetic Techniques for Gibberella zeae

Leslie, J. F. 1983. Some genetic techniques for Gibberella zeae. Phytopathology 73:1005-1008. Gibberella zeae, an important plant pathogen, is exploited commercially dependent, glutamate dehydrogenase-deficient mutant. Protoplasts were in the production of zearalenone, a fungal sex hormone. Genetically, G. liberated by hyphal digestion with commercially available f-glucuronidase zeae is relativ...

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Plump kernels with high deoxynivalenol linked to late Gibberella zeae infection and marginal disease conditions in winter wheat.

Deoxynivalenol (DON) concentrations in mature wheat grain are usually correlated with symptoms produced by Gibberella zeae infection. However, there have been numerous observations of unacceptably high DON in asymptomatic crops, which can lead to lower-than-expected milling reductions in DON. We conducted a field experiment with winter wheat to examine the effect of infection timing and postant...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1990

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.56.10.3047-3051.1990